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目錄號(hào): 100103

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您的評(píng)價(jià): Anti-h PSA 8311 SPRN-1
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Liquid, may turn slightly opaque during stroage

Human

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產(chǎn)品概述
產(chǎn)品名稱 Anti-h PSA 8311 SPRN-1
目錄號(hào) 100103
描述 Monoclonal mouse antibody, cultured in vitro under conditions free from animal-derived components.
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補(bǔ)充說(shuō)明
劑型/外觀 Liquid, may turn slightly opaque during stroage
濃度 1.0–1.1 mg/ml
備注 Clone 8311 reacts with non-reduced and reduced intact PSA but not with reduced PSA fragments (Rye et al., 1999). In the same study affinities for free-PSA and PSA-ACT-complex were, 1.7 x 10-10 mol/l and 1.55 x 10-10, respectively. They also noted that antibodies binding to epitope group 6 form a good pair with antibodies from groups 1 and 3. Clone 8311 crossreacts with human kallikrein-2 (Black, 1999b) and can be used to develop a hK2-assay.
抗體亞型 IgG2a
克隆性質(zhì) Monoclonal
抗原決定簇 Residues 3–11, group 6a as described in Rye et al. (1999)
純度 ≥ 95 %
親和常數(shù) KA= 1 x 1010 1/M
相關(guān)產(chǎn)品 Native PSA antigen, Lee Biosolutions 497-11 and 497-17
緩沖液 37 mM citrate, 125 mM phosphate, pH 6.0, 0.9 % NaCI, 0.095 % NaN3 as a preservative
等電聚焦圖譜 6.8–7.5
交叉反應(yīng) PSA/α1-ACT complex 76 %, Kallikrein < 0.36 %, PAP < 0.36 %
特異性 Antibody recognizes human prostate-specific antigen
儲(chǔ)運(yùn)條件
儲(chǔ)存條件 +2-8°C
運(yùn)輸條件 Cold packs
庫(kù)存及貨期 小包裝樣品現(xiàn)貨
有效期 36 months
參考文獻(xiàn)
文獻(xiàn) Black, M.H., Grass, C.L., Leinonen, J., Stenman, U-H. and Diamandis, E.P., (1999a) Characterization of monoclonal antibodies for prostate-specific antigen and development of highly sensitive free prostate-specific antigen assays. Clin. Chem., 45(3):347-354 Black, M.H., Magklara, A., Obiezu, C.V., Melegos, D.N. and Diamandis, E.P., (1999b) Development of an ultrasensitive immuoassay for human glandular kallikrein with no crossreactivity from prostate-specific antigen. Clin. Chem., 45(6):790-799 Ferguson, R.A., Yu, H., Kalyvas, M., Zammit, S. and Diamandis, E.P., (1996) Ultrasensitive detection of prostate-specific antigen by time-resolved immunofluorometric assay and the Immulite? immunochemiluminescent third-generation assay: potential applications in prostate and breast cancer. Clin. Chem., 42(5):675-684 Magklara, A., Scorilas, A., López-Otín, C., Vizoso, F., Ruibal, A. and Diamandis, E.P., (1999) Human glandular kallikrein in breast milk, amniotic fluid, and breast cyst fluid. Clin. Chem., 45(10):1774-1780 Obiezu, C.V., Giltay, E.J., Magklara, A., Scorilas, A., Gooren, L.J.G., Yu, H., Howarth, D.J.C. and Diamandis, E.P., (2000) Serum and urinary prostate-specific antigen and urinary human glandular kallikrein concentrations are significantly increased after testosterone administration in female-to-male transsexuals. Clin. Chem., 46(6):859-862 Rye, P.D., Bormer, O.P. and Paus, E. (guest editors) (1999), ISOBM TD-3 International Workshop on monoclonal antibodies against prostate-specific antigen. Tumor Biol., 20(suppl 1):1-94
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